期刊
REGULATORY PEPTIDES
卷 134, 期 1, 页码 17-22出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.regpep.2005.11.001
关键词
GHS-R1(a); adipose tissue; des-acyl ghrelin
资金
- Medical Research Council [G7811974, G108/535] Funding Source: researchfish
- MRC [G7811974, G108/535] Funding Source: UKRI
- Medical Research Council [G108/535, G7811974] Funding Source: Medline
The gastric and hypothalamic hormone ghrelin is the endogenous agonist of the growth hormone secretagogue receptor GHS-R1(a). Ghrelin stimulates growth hormone release and appetite via the hypothalamus. However, putative direct peripheral effects of ghrelin remain poorly understood. Rat adipose tissue expresses GHS-R1(a) mRNA, suggesting ghrelin may directly influence adipocyte function. We have investigated the effects of ghrelin oil insulin-stimulated glucose uptake in isolated white adipocytes in vitro. RT-PCR confirmed the expression of GHS-R1(a) mRNA in epididymal adipose tissue. However, GHS-R1(a) expression was not detected in the peri-renal fat pads. Ghrelin increased insulin-stimulated deoxyglucose uptake in isolated white adipocytes extracted from the epididymal fat pads of male Wistar rats. Ghrelin 1000 nM significantly increased deoxyglucose uptake by 55% in the presence of 0.1 nM insulin. However, ghrelin administration in the absence of insulin had no effect on adipocyte deoxyglucose uptake, suggesting that ghrelin acts synergistically with insulin. Des-acyl ghrelin, a major circulating non-octanylated form of ghrelin, had no effect oil insulin-stimulated glucose uptake. Furthermore, acylated ghrelin had no effect oil deoxyglucose uptake in adipocytes from peri-renal fat pads Suggesting that ghrelin may influence glucose uptake via the GHS-R1(a). Ghrelin therefore appears to directly potentiate adipocyte insulin-stimulated glucose uptake in selective adipocyte Populations. Ghrelin may play a role in adipocyte regulation Of glucose homeostasis. (C) 2005 Elsevier B.V. All rights reserved.
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