期刊
TALANTA
卷 69, 期 1, 页码 210-215出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2005.09.029
关键词
poly(dimethylsiloxane); microchip electrophoresis; gold nanoparticles; dopamine; epinephrine
Poly(dimethylsiloxane) (PDMS) microfluidic channels modified by citrate-stabilized gold nanoparticles after coating a layer of linear polyethylenimine (LPEI) were successfully used to separate dopamine and epinephrine, which were difficult to be separated from baseline in native and hybrid PDMS microchannels. In-channel amperometric detection with a single carbon fibre cylindrical electrode was employed. Experimental parameters of separation and detection processes were optimized in detail. The analytes were well separated within 100 s in a 3.7 cm long separation channel at a separation voltage of +800 V using a 30 mM phosphate buffer solution (PBS, pH 7.0). Linear responses of them were obtained both from 25 to 600 mu M with detection limits of 2 mu M for dopamine and 5 mu M for epinephrine, respectively. The modified PDMS channels have a long-term stability and an excellent reproducibility within 2 weeks. (c) 2005 Elsevier B.V. All rights reserved.
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