期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 40, 期 5, 页码 1225-1230出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2005.03.047
关键词
FT-Raman spectroscopy; captopril; prednisolone; pharmaceuticals; quantitative determination
A procedure for the quantitative determination of captopril and prednisolone in commercial tablets based on partial least squares (PLS) and principal component regression (PCR) treatment of FT-Raman spectroscopic data is described. In the studied medicines active pharmaceutical ingredients (APIs) constitute 4.2-16.7% of the tablet mass. Results obtained from calibration models built using unnormalised spectra were compared with the values found when an internal standard was added to each sample and the spectra were normalised by its selected band intensity at maximum or integrated. To apprise the quality of the models the relative standard error of predictions (RSEPs) were calculated for calibration and testing data sets. For captopril determination these were 1.8-2.2% (2.1-2.3%) and 2.7-3.1% (2.7-3.6%), respectively for the different PLS (PCR) models. For prednisolone these errors amounted to 1.8-2.1% (2.6-3.5%) and 3.2-3.7% (3.7-5.9%), respectively. Three commercial preparations of captopril containing 12.5 mg and one 25 mg of API per tablet were quantified using developed models. Found captopril contents, calculated versus results of iodometric titration, was equal 99.2-101.2% (99.2-102.0%), for the different PLS (PCR) calibration models and the different preparations. Quantification of prednisolone tablets, declared content 5 mg per tablet, on the basis of PLS (PCR) models gave API amount, calculated versus results of UV-vis method, in the 99.0-101.0% (98.0-102.0%) range. (c) 2005 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据