期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 534, 期 1-3, 页码 39-47出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2006.01.025
关键词
chemotaxis; 17-beta-estradiol; estrogen receptor; transforming growth factor-beta; Smad proteins; breast cancer cell
Motility of malignant cells plays a crucial role for the metastasis of tumours. Both, 17-beta-estradiol and transforming growth factor-beta TGF-beta, induce migration of MCF-7 breast cancer cells and simultaneous treatment resulted in an additive effect of the migratory response. But most interestingly, when cells were preincubated with 17-beta-estradiol, the ability of TGF-beta to evoke chemotaxis was drastically diminished. Abrogation of Smad signalling indicated that this pathway is essential for TGF-beta-mediated MCF-7 cell migration. In agreement, pretreatment of MCF-7 cells with 17-beta-estradiol resulted in a reduced phosphorylation of Smad2 and Smad3 as well as a diminished Smad2 and Smad3 gene reporter activity in response to TGF-beta. Thus, these results indicate a controversial role of 17-beta-estradiol on MCF-7 cell migration. 17-beta-estradiol potently increases the migratory potency of MCF-7 cells, but inhibits TGF-beta-induced migration by an interaction between estrogen receptors and Smad proteins. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据