期刊
FEBS LETTERS
卷 580, 期 7, 页码 1795-1800出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.02.035
关键词
filamin A; siRNA; Ca2+-sensing receptor; signaling; MAP kinase
资金
- NHLBI NIH HHS [HL-41618] Funding Source: Medline
- NIDDK NIH HHS [DK-59985] Funding Source: Medline
Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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