期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 114, 期 1, 页码 445-450出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2005.06.021
关键词
biosensor; Si; alkyl monolayer; SAMs; FTIR-ATR; DNA; oligonucleotide; polyamide; photocleavage; aptamer; thrombin
With the help of chemical modification of silicon surfaces, FTIR-ATR was developed into a biosensing method to monitor biochemical processes on surfaces. In this proof-of principle work, FTIR-ATR crystals were covalently modified by oligonucleotides and two model systems were investigated, including recognition of anti-thrombin aptamer with thrombin and organic molecule binding to double helix DNA with sequence-specific photocleavage. The affinity of aptamer recognition and the affinity of photocleavage reagent towards double helix of DNA were quantitatively measured. Thus, it was shown that the proposed FTIR-ATR-based biosensing method allows for high-sensitive label-free detection of various biological systems. (c) 2005 Elsevier B.V. All rights reserved.
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