期刊
SCIENCE
卷 311, 期 5769, 页码 1887-1893出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1122771
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资金
- NCI NIH HHS [CA31760-24] Funding Source: Medline
- NIGMS NIH HHS [GM56312] Funding Source: Medline
Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran. Depletion of lamin B resulted in defects in spindle assembly. Dominant negative mutant lamin B proteins that disrupt lamin B assembly in interphase nuclei also disrupted spindle assembly in mitosis. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis.
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