期刊
EXPERIMENTAL PARASITOLOGY
卷 112, 期 4, 页码 232-236出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2005.11.008
关键词
blood meal; DNA; PCR; mosquito; post-ingestion; physical condition
类别
The effects of post-ingestion and physical conditions under which killed mosquitoes are stored on the success of detecting blood meal DNA of Anopheles stephensi and Culex quinquefasiatus were investigated by polymerase chain reaction (PCR) amplification at the human mitochondrial DNA cytochrome b (cytB) gene. I-lost DNA extracted from the blood meal Lip to 33 h post-ingestion in both species acts as,in efficient template for PCR amplification. However, more DNA concentration is needed for meals digested for a longer time, and successful PCR amplification from meals digested for 36h,dropped to a faint band. There were no differences between PCR Success rate for samples stored at +4 or -20 degrees C, but less Successful products were observed in samples kept at 4 degrees C for the periods longer than 30 h digestion. The results of this Study are important for conducting malaria epidemiological Studies that provide information about the degree of contact between human hosts and mosquito vectors, impact of vector controls such as bed nets and repellents, and the transmission dynamics of human malaria and other vector-borne diseases. (c) 2005 Elsevier Inc. All rights reserved.
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