期刊
EUKARYOTIC CELL
卷 5, 期 4, 页码 723-731出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.5.4.723-731.2006
关键词
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资金
- NIGMS NIH HHS [R01 GM050403, GM50403] Funding Source: Medline
Phosphoinositide lipids regulate complex events via the recruitment of proteins to a specialized region of the membrane at a specific time. Precise control of both the synthesis and turnover of phosphoinositide lipids is integral to membrane trafficking, signal transduction, and cytoskeletal rearrangements. Little is known about the acute regulation of the levels of these signaling lipids. When Saccharomyces cerevisiae cells are treated with hyperosmotic medium the levels of phosphatidylinositol 3,5-bisphosphate (P13,5P(2)) increase 20-fold. Here we show that this 20-fold increase is rapid and occurs within 5 min. Surprisingly, these elevated levels are transient. Fifteen minutes following hyperostnotic shock they decrease at a rapid rate, even though the cells remain in hyperosmotic medium. In parallel with the rapid increase in the levels of P13,5P(2), vacuole volume decreases rapidly. Furthermore, concomitant with a return to basal levels of P13,5P(2) vacuole volume is restored. We show that Fig4p, consistent with its proposed role as a P13,5P(2) 5-phosphatase, is required in vivo for this rapid return to basal levels of P13,5P(2). Surprisingly, we find that Fig4p is also required for the hyperosmotic shock-induced increase in P13,5P(2) levels. These findings demonstrate that following hyperosmotic shock, large, transient changes occur in the levels of P13,5P(2) and further suggest that Fig4p is important in regulating both the acute rise and subsequent fall in P13,5P(2) levels.
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