期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 44, 期 4, 页码 1519-1529出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.44.4.1519-1529.2006
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资金
- NIAID NIH HHS [R01 AI051292, U54 AI057158, AI 51292, U54 AI57158] Funding Source: Medline
Here we propose the use of a 216-nucleotide fragment located in the carboxyl terminus of the E gene (E-COOH) and a pairwise-based comparison method for genotyping of dengue virus 1 (DENV-1) strains. We have applied this method to the detection and characterization of DENV-1 in serum samples from travelers returning from the tropics. The results obtained with the typing system correlate with the results obtained by comparison of the sequences of the entire E gene of the strains. The approach demonstrates utility in plotting the distribution and circulation of different genotypes of DENV-1 and also suggests the presence of two new clades of Indian strains. The integration of the method with an online database and a typing characterization tool enhances its strength. Additionally, the analysis of the complete E gene of DENV-1 strains suggested the occurrence of a nondescribed recombination event in the China GD23-95 strain. We propose the use of this methodology as a tool for real-time epidemiological surveillance of dengue virus infections and their pathogenesis.
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