期刊
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
卷 60, 期 4, 页码 736-743出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/01.ta.0000196802.91829.cc
关键词
sympathetic nervous system; norepinepbrine; wound healing; inflammation; angiogenesis; innate immunity
资金
- NIGMS NIH HHS [R01-GM55238, R01 GM050875, R01-GM50875] Funding Source: Medline
Background: Injury results in the massive release of norepinephrine (NE) into the peripheral circulation. Recent investigations have demonstrated functional adrenoreceptors on the cellular mediators of cutaneous wound healing and NE-induced phenotypic alterations in immune cells have been demonstrated in vitro. Despite this, there is little description of how NE might alter the phases of wound healing in vivo. The purpose of this study was to compare cutaneous wound heating in norepinephrine-intact and norepinephrine-depleted mice. Methods: Norepinephrine-depleted (NED) mice were generated by chemical axotomy with 6-hydroxydopamine and compared with norepinephrine-intact (NEI) animals (n = 6-12 per group, per time point). Using an excisional wound model, neutrophil recruitment was measured by myeloperoxidase assay. Macrophage recruitment and angiogenesis were measured by immunohistochemistry and reepithelialization was determined histologically. The development of incisional wound disruption strength was determined over time. Finally, macrophage scavenger function was assessed by an in vitro latex bead phagocytosis assay. Results: Wounds from NEI mice demonstrated greater neutrophil infiltration than NED wounds (24, 72 hours; p < 0.05). Wound macrophage recruitment was initially higher in NEI animals (24 hours, p < 0.05), but was eventually surpassed by that of NED animals (120 hours, p < 0.05). Angiogenesis was decreased while re-epithelialization was accelerated in NEI animals (P < 0.05). Wound disruption strength and macrophage scavenger function were unaltered between NED and NEI mice. Conclusions: Norepinephrine modulates the inflammatory and proliferative phases of wound healing in a temporally defined, cell-specific manner. By increasing recruitment of innate immune cells and expediting wound closure, norepinephrine appears to play a protective role in defense against infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据