期刊
METHODS
卷 38, 期 4, 页码 283-293出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2005.11.007
关键词
Western blotting; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; nitrocellulose; polyvinylidene difluoride membrane; detection techniques
资金
- NIAMS NIH HHS [AR01844] Funding Source: Medline
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer. This review describes the various procedures that have been used to transfer proteins from a gel to a membrane based on the principles of simple diffusion, vacuum-assisted solvent flow and electrophoretic elution. Finally, a brief description of methods generally used to detect antigens on blots is also described. (c) 2006 Elsevier Inc. All rights reserved.
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