期刊
BIOTECHNOLOGY LETTERS
卷 28, 期 7, 页码 477-483出版社
SPRINGER
DOI: 10.1007/s10529-006-0002-y
关键词
Escherichia coli; human soluble B lymphocyte stimulator; lactose induction
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l(-1) and a productivity of 0.11 g l(-1) h(-1).
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