期刊
ANALYTICAL BIOCHEMISTRY
卷 351, 期 1, 页码 18-25出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.01.038
关键词
resouance light scattering; gold nanoparticles; cysteine
The interaction between cysteine and gold nanoparticles was studied. Through the covalent combination with the -SH group and the electrostatic binding with the -NH3+ group of cysteine, gold nanoparticles call self-assemble to form a network Structure, Which results in greatly enhanced resonance light scattering (RLS). The experimental results demonstrate that the RLS technique offers a sensitive tool for investigations of self-assembly of nanoparticles. On the other hand, the RLS method can be applied to selectively determine cysteine with high sensitivity and simple operation. The linear range of determination of cysteine is from 0.01 to 0.25 mu g/mL with the detection limit of 2.0 ng/mL (16.5 nM, 3 sigma). None of the amino acids found in proteins inteferes with the determination. (c) 2006 Elsevier Inc. All rights reserved.
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