期刊
PROTEOMICS
卷 6, 期 7, 页码 2135-2146出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200500512
关键词
high-throughput strain; protein complexes; protein interaction; subcellular localisation
Construction and microscopic imaging of protein fusions to green fluorescent protein (GFP) have revolutionised our understanding of bacterial structure and function. We have undertaken a systematic study of the localisation of over 100 Bacillus subtilis proteins, following the development of high-throughput construction and analysis procedures. We focused on proteins linked in various ways to the DNA replication machinery, as well as on proteins exemplifying a range of other cellular functions and structures. The results validate the approach as a way of obtaining systematic protein localisation information. They also provide a range of novel biological insights, particularly through the identification of a number of proteins not previously known to be associated with the DNA replication factory.
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