期刊
HUMAN IMMUNOLOGY
卷 67, 期 4-5, 页码 298-302出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2006.02.031
关键词
BK virus; immunity; large T antigen
类别
资金
- NIAID NIH HHS [AI 063660, R0-1 AI 51227] Funding Source: Medline
BK virus (BKV) infections after renal transplantation are increasingly recognized. Development of immune monitoring strategies against BKV requires definition of antigenic epitopes. Hence, T cells from HLA-A02-positive healthy subjects and kidney transplant recipients were stimulated by BKV lysate pulsed on mature autologous dendritic cells and screened against four different T antigen peptides or against BKV lysate. IFN-gamma production was measured by ELISPOT assays. The peptide BKV362-371 (MLTERFNHIL) was naturally processed and recognized by five of six healthy subjects (39 +/- 11 IFN-gamma spots/100,000 cells) and five of seven kidney transplant recipients (21 +/- 12 IFN-gamma spots). Less frequent and weaker CD8(+) T-cell responses were detected against three other peptides. Thus, BKV large T antigen is a target for CD8(+) T-cell immunity. T-antigen-specific T-cytotoxic cells circulate in healthy blood donors, implying that transient expression of T antigen presumably occurs at sites of viral latency and helps maintain a constant pool of circulating CD8(+) T memory cells.
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