期刊
STRUCTURAL CHEMISTRY
卷 17, 期 2, 页码 163-175出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11224-006-9025-1
关键词
agmatine; physiological structure; mitochondrial permeability transition; DFT calculations; Raman spectroscopy
The present work aims at determining the structure-activity relationships (SAR's) which rule the biological function of agmatine (4-(aminobutyl)guanidinium, AGM), a biogenic amine produced by decarboxylation of arginine. Its structural preferences, both as an isolated molecule and in aqueous solution (namely at physiological conditions) were ascertained, by vibrational (Raman) spectroscopy coupled to theoretical (density functional) calculations. An evaluation of mitochondrial functions (membrane potential (Delta Psi), mitochondrial swelling, and cytochrome c release) in rat liver mitochondria (RLM) was also carried out. The results thus obtained, coupled to the conformational analysis performed for the distinct polyamine protonation states, allowed to individualize the agmatine structures which interact with the mitochondrial site responsible for its transport and for the protection against mitochondrial permeability transition (MPT) induction, as well as to gain information on the specific mechanisms involved.
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