期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 290, 期 4, 页码 C1199-C1208出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00469.2005
关键词
skeletal muscle; low-frequency fatigue; ryanodine receptor; skinned fiber
The repeated elevation of cytosolic Ca2+ concentration ([Ca2+](i)) above resting levels during contractile activity has been associated with long-lasting muscle fatigue. The mechanism underlying this fatigue appears to involve elevated [Ca2+](i) levels that induce disruption of the excitation-contraction (E-C) coupling process at the triad junction. Unclear, however, are which aspects of the activity-related [Ca2+](i) changes are responsible for the deleterious effects, in particular whether they depend primarily on the peak [Ca2+](i) reached locally at particular sites or on the temporal summation of the increased [Ca2+] in the cytoplasm as a whole. In this study, we used mechanically skinned fibers from rat extensor digitorum longus muscle, in which the normal E-C coupling process remains intact. The [Ca2+](i) was raised either by applying a set elevated [Ca2+] throughout the fiber or by using action potential stimulation to induce the release of sarcoplasmic reticulum Ca2+ by the normal E-C coupling system with or without augmentation by caffeine or buffering with BAPTA. Herein we show that elevating [Ca2+](i) in the physiological range of 2-20 mu M irreversibly disrupts E-C coupling in a concentration-dependent manner but requires exposure for a relatively long time (1-3 min) to cause substantial uncoupling. The effectiveness of Ca2+ released via the endogenous system in disrupting E-C coupling indicates that the relatively high [Ca2+](i) attained close to the release site at the triad junction is a more important factor than the increase in bulk [Ca2+](i). Our results suggest that during prolonged vigorous activity, the many repeated episodes of relatively high triadic [Ca2+] can disrupt E-C coupling and lead to long- lasting fatigue.
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