期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 14, 页码 5332-5337出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0600828103
关键词
nonspecific DNA binding; gene regulation promoter; systems biology; Escherichia coli
资金
- NIGMS NIH HHS [R37 GM036278, R01 GM036278, GM 36278-18] Funding Source: Medline
To explore scenarios that permit transcription regulation by activator recruitment of RNA polymerase and or competition in vivo, we used an equilibrium model of RNA polymerase binding to DNA constrained by the values of total RNA polymerase (E) and sigma(70) per cell measured in this work. Our numbers of E and sigma(70) per cell, which are consistent with most of the primary data in the literature, suggest that in vivo (i) only a minor fraction of RNA polymerase (< 20%) is involved in elongation and (ii) sigma(70) is in excess of total E. Modeling the partitioning of RNA polymerase between promoters, nonspecific DNA binding sites, and the cytoplasm suggested that even weak promoters will be saturated with E sigma(70) in vivo unless nonspecific DNA binding by E sigma(70) is rather significant. In addition, the model predicted that sigma s compete for binding to E only when their total number exceeds the total amount of RNA polymerase (excluding that involved in elongation) and that weak promoters will be preferentially subjected to sigma competition.
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