期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1111, 期 2, 页码 220-227出版社
ELSEVIER
DOI: 10.1016/j.chroma.2005.07.083
关键词
ATP; erythrocytes; microchip; PDMS; chemiluminescence
资金
- NIBIB NIH HHS [R01-EB00416401] Funding Source: Medline
- PHS HHS [R01-073942] Funding Source: Medline
The release of adenosine triphosphate (ATP) from red blood cells (RBCs) flowing through PDMS microchannels has been determined as a function of channel cross-sectional area using a design containing a channel that narrows uniformly. ATP, released from the RBCs in response to the mechanical deformation of their cell membranes, increased as the channel cross-section decreased. One sample of rabbit RBCs released 1.16 +/- 0.11, 1.92 +/- 0.14 and 2.09 +/- 0.10 mu M ATP as the median cross-sectional area decreased from 4314 to 3192 to 2052 mu m(2), respectively. Numerous samples (n=6) displayed the same trend. Incubating a sample of RBCs with diamide, a substance known to stiffen cell membranes without harming the cell cytosol, provided evidence that no cell lysis occurred in the microchip device. This novel use of lab-on-a-chip technology allows for channel designs that enable an in vitro study of physiological events that occur in the microcirculation. (c) 2005 Elsevier B.V. All rights reserved.
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