期刊
IMMUNOLOGY LETTERS
卷 104, 期 1-2, 页码 124-130出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2005.11.011
关键词
cortactin; integrin; SH3 domain; siRNA; cell spreading
类别
资金
- Wellcome Trust Funding Source: Medline
Cortactin is an SH3 domain-containing protein that contributes to the formation of dynamic cortical actin-associated structures, such as lamellipodia and membrane ruffles. Here we show that expression of either the GFP-tagged N-terminal or the C-seminal halves of cortactin inhibits significantly the spreading of COS7 cells on fibronectin. Introducing inactivating point mutation into the SH3 domain of the C-terminal half of cortactin suspends the dominant negative effect of the construct. In addition, a vector-based RNA interference was used to knock-down endogenous level of cortactin in cells. We demonstrate that cortactin deficient cells were not able to spread. These results suggest that cortactin is required for integrin-mediated signalling pathways. (C) 2005 Elsevier B.V All rights reserved.
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