期刊
CELL CYCLE
卷 5, 期 8, 页码 829-830出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.5.8.2686
关键词
apoptosis; natively unstructured protein; p8; prothymosin alpha; caspases; two hybrid system; protein complex
类别
p8 and prothymosin a are two natively unstructured proteins with anti-apoptotic activity. We showed that their interaction results in the formation of a one-to-one heterodimer complex with stable structure. To test whether the heterodimer bears the function previously attributed to both proteins, we monitored the consequences on apoptosis of modulating in vitro the concentrations of both proteins. Overexpression was obtained by transfection of appropriate vectors and inhibition by using specific siRNAs. In all conditions inhibition of apoptosis correlated with the level of the partner with lowest concentration, demonstrating that the anti-apoptotic effect previously attributed to each proteins was in fact borne by the p8/ProT alpha complex, the two proteins, being individually inactive. These results show that the function attributed to a natively unfolded protein might actually belong to a multi- protein complex in which the protein of interest is engaged.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据