期刊
DEVELOPMENT
卷 133, 期 8, 页码 1485-1494出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.02334
关键词
Drosophila; Moleskin; Importin 7; morphogenetic furrow; MAP kinase; cell cycle; nuclear translocation; ERK; Hedgehog; Dpp; Egfr
资金
- NEI NIH HHS [R01 EY12537, R01 EY012537] Funding Source: Medline
- NICHD NIH HHS [P01 HD39942, P01 HD039942] Funding Source: Medline
- NIGMS NIH HHS [F32 GM073608-01A1, T32 GM008490, 1 F32 GM073608, R01 GM61707, F32 GM073608, R01 GM061707] Funding Source: Medline
The Drosophila Mitogen Activated Protein Kinase ( MAPK) Rolled is a key regulator of developmental signaling, relaying information from the cytoplasm into the nucleus. Cytoplasmic MEK phosphorylates MAPK (pMAPK), which then dimerizes and translocates to the nucleus where it regulates transcription factors. In cell culture, MAPK nuclear translocation directly follows phosphorylation, but in developing tissues pMAPK can be held in the cytoplasm for extended periods ( hours). Here, we show that Moleskin antigen ( Drosophila Importin 7/Msk), a MAPK transport factor, is sequestered apically at a time when lateral inhibition is required for patterning in the developing eye. We suggest that this apical restriction of Msk limits MAPK nuclear translocation and blocks Ras pathway nuclear signaling. Ectopic expression of Msk overcomes this block and disrupts patterning. Additionally, the MAPK cytoplasmic hold is genetically dependent on the presence of Decapentaplegic (Dpp) and Hedgehog receptors.
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