期刊
FEBS LETTERS
卷 580, 期 9, 页码 2238-2246出版社
WILEY
DOI: 10.1016/j.febslet.2006.03.035
关键词
membrane fusion; SNARES; hemifusion; synaptotagmin; kiss-and-run
Synaptic membrane fusion, which is necessary for neurotransmitter release, may be mediated by SNARES and regulated by synaptotagmin (Syt) and Ca2+. Fusion of liposomes mediated by reconstituted SNARES produces full fusion and hemifusion, a membrane structure in which outer leaflets are mixed but the inner leaflets remain intact. Here, using the liposome fusion assay, it is shown that Syt promoted both hemifusion and full fusion in a Ca2+-dependent manner. Syt center dot Ca2+ increased hemifusion more than full fusion, modulating the ratio of hemifusion to full fusion. Unlike the case of neuronal SNARES, stimulation of fusion by Syt center dot Ca2+ was not seen for other SNARES involved in traffijking in yeast, indicating that the Syt center dot Ca2+ stimulation was SNARE-specific. We constructed hybrid SNARES in which transmembrane domains were swapped between neuronal and yeast SNARES. With these hybrid SNARES, we demonstrated that the interaction between the SNARE motifs of neuronall proteins and Syt center dot Ca2+ was required for the stimulation of fusion. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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