期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 93, 期 6, 页码 1130-1137出版社
WILEY
DOI: 10.1002/bit.20833
关键词
gradient; enzyme; scaffolds; 3D; immobilize; diffusion
资金
- NIBIB NIH HHS [EB00321, EB002520] Funding Source: Medline
Horseradish peroxide (HRP) was covalently coupled to three-dimensional (3D) silk fibroin scaffolds using water-soluble carbodiimide. Stable, bilaterally symmetrical immobilized HRP gradient patterns were generated within 3D silk fibroin scaffolds using the principles of diffusion. Gradients of immobilized HRP activity were controlled using variables of volume and concentration of HRP solution activated by the carbodiimide. The method developed can be extended to immobilize a variety of proteins and small molecules on several types of porous, interconnected materials. This technique of patterning enzymes and proteins in a gradient manner offers new options in the field of chemotaxis, tissue engineering, and biosensors. (c) 2006 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据