期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 108, 期 2, 页码 204-209出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2005.12.004
关键词
Aspergillus; grape; epiphytic yeast; growth inhibition; biocontrol agents
Antagonistic yeasts were isolated from the epiphytic flora associated with grape berries cv. Negroamaro and identified at species level using molecular methods. A total of 144 yeast isolates were tested in a preliminary screening on agar to select isolates showing a killer activity against Aspergillus carbonarius and A. niger, the main species responsible for the accumulation of ochratoxin A in grape. Twenty-eight yeast isolates were selected for their inhibitory effects on the above fungal species and assayed by an in vitro nutritional competition test for their antagonistic capacity towards three selected ochratoxigenic strains. Six yeast isolates belonging to five species, namely 2 isolates of Issatchenkia orientalis and one each of Metschnikowia pulcherrima, Kluyveromyces thennotolerans, Issatchenkia, terricola and Candida incommunis, were finally selected and screened on wounded grape berries for their ability to inhibit infection by ochratoxigenic moulds. With the exception of the K. thermotolerans isolate, when inoculated at 10(9) CFU/wound, the other five challenger yeasts reduced the A. carbonarius and A. niger colonization on grape betty (P < 0.05). In particular, the best antagonistic activity was shown by the two L. orientalis isolates. Results suggest that antagonist yeasts with the potential to control A. carbonarius and A. niger on grape can be found among the microflora associated with the berries. (c) 2005 Elsevier B.V. All rights reserved.
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