期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 343, 期 1, 页码 159-166出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.02.127
关键词
embryonic stein cell; self-renewal; Wnt; LIF; beta-catenin; BIO
Leukaemia inhibitory factor (LIF) was the first. soluble factor identified as having potential to maintain the pluripotency of mouse embryonic stein (ES) cells. Recently, a second factor. Wilt, with similar activity was found. However, the relationship between these completely different signals mediating the overlapping functions is still unclear. Here, we report that the conditioned medium oft-cells expressing Wnt3a maintains ES cells in the undifferentiated state in feeder-free culture, followed by expression of stein cell markers and their ability to generate germline chimaeras. However, although the activity of this conditioned medium is dependent on Wnt3a, recombinant Wnt3a protein cannot maintain ES cells in the undifferentiated state. As supplementation with Wnt3a to the sub-threshold level of LIF alone was not sufficient to maintain ES self-renewal, the results of maintenance of the undifferentiated state indicated the synergistic action of Wnt and LIF. Induction of constitutively activated beta-catenin alone is unable to maintain ES self-renewal but shows a synergistic effect with LIF. These observations indicate that the Wilt signal mediated by the canonical pathway is not sufficient but enhances the effect of LIF to maintain self-renewal of mouse ES cells. (c) 2006 Elsevier Inc. All rights reserved.
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