期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 11, 期 3, 页码 268-272出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/BF02932042
关键词
hydrodynamic stability; protein adsorption; expanded bed chromatography; bovine serum albumin; yeast cells
A dense, pellicular UpFront adsorbent (rho = 1.5 g/cm(3), UpFront Chromatography, Cophenhagen, Denmark) was characterized in terms of hydrodynamic properties and protein adsorption performance in expanded bed chromatography. Cibacron Blue 3GA was immobilised into the adsorbent and protein adsorption of bovine serum albumin (BSA) was selected to test the setup. The Bodenstein number and axial dispersion coefficient estimated for this dense pellicular adsorbent was 54 and 1.63 X 10(-5) m(2)/s, respectively, indicating a stable expanded bed. It could be shown that the BSA protein was captured by the adsorbent in the presence of 30% (w/v) of whole-yeast cells with an estimated dynamic binding capacity (C/C-o = 0.01) of approximately 6.5 mg/mL adsorbent.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据