期刊
ANNALS OF PLASTIC SURGERY
卷 69, 期 6, 页码 650-655出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SAP.0b013e3182745f95
关键词
hyperbaric oxygen; gelatin/polycaprolactone; cartilage tissue engineering; human adipose-derived stem cells (hASCs); glycosaminoglycan; atmosphere absolute (ATA)
类别
There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development.
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