期刊
MICROVASCULAR RESEARCH
卷 71, 期 3, 页码 218-221出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mvr.2006.02.003
关键词
troponin I-like molecule; Haemaphysalis longicornis; angiogenesis; VEGF
This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (H-UVEC) in vitro. The inhibition was dose-dependent with an IC50 of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor. (c) 2006 Elsevier Inc. All rights reserved.
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