Chemiluminescence determination of metformin based on hydroxyl radical reaction and molecularly imprinted polymer on-line enrichment

A novel and simple chemiluminescence (CL) method has been developed and validated for determination of metformin. This method is based on hydroxyl radical chemiluminescence - the hydroxyl radical generated by reaction of Cu(II) and hydrogen peroxide oxidizes rhodamine B (RhB) to produce weak CL which can be enhanced by metformin. At the same time, metformin molecularly imprinted polymer (MIP) was synthesized. After enrichment based on the selectivity of metformin-MIP, the CL method was successfully applied to the determination of metformin in human serum. The linear range was from 1.0 x 10(-8) to 1.0 x 10(-6) g mL(-1) and the detection limit was 4 x 10(-9) g mL(-1). The relative standard deviation at 2.0 x 10(-7) g mL(-1) by use of MIP was 3.67% (n= 7).