期刊
PROTEOMICS
卷 6, 期 9, 页码 2650-2655出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200500654
关键词
O-6-alkylguanine-DNA-alkyltransferase; protein array; protein-DNA-conjugate; self-assembly; SNAP-tag (TM)
The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O-6-alkylguanine-DNA-alkyltransferase (SNAP-tag (TM)) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.
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