期刊
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
卷 317, 期 2, 页码 694-703出版社
AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/jpet.105.098319
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资金
- Intramural NIH HHS Funding Source: Medline
- NIMH NIH HHS [MH-64797] Funding Source: Medline
Androstenol is a steroidal compound belonging to the group of odorous 16-androstenes, first isolated from boar testes and also found in humans. Androstenol has pheromone-like properties in both animals and humans, but the molecular targets of its pheromonal activity are unknown. Androstenol is structurally similar to endogenous A-ring reduced neurosteroids that act as positive modulators of GABA A receptors. Here we show that androstenol has neurosteroid-like activity as a GABA A receptor modulator. In whole-cell recordings from cerebellar granule cells, androstenol ( but not its 3 beta-epimer) caused a concentration-dependent enhancement of GABA- activated currents ( EC50, 0.4 mu M in cultures; 1.4 mu M in slices) and prolonged the duration of spontaneous and miniature inhibitory postsynaptic currents. Androstenol ( 0.1-1 mu M) also potentiated the amplitude of GABA-activated currents in human embryonic kidney 293 cells transfected with recombinant alpha 1 beta 2 gamma 2 and alpha 2 beta 2 gamma 2 GABA A receptors and, at high concentrations ( 10-300 mu M), directly activated currents in these cells. Systemic administration of androstenol ( 30-50 mg/kg) caused anxiolytic-like effects in mice in the open-field test and elevated zero-maze and antidepressant-like effects in the forced swim test ( 5-10 mg/ kg). Androstenol, but not its 3 beta-epimer, conferred seizure protection in the 6-Hz electroshock and pentylenetetrazol models ( ED50 values, 21.9 and 48.9 mg/ kg, respectively). The various actions of androstenol in the whole-animal models are consistent with its activity as a GABA A receptor modulator. GABA A receptors could represent a target for androstenol as a pheromone, for which it is well suited because of high volatility and lipophilicity, or as a conventional hormonal neurosteroid.
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