期刊
JOURNAL OF PROTEOME RESEARCH
卷 5, 期 5, 页码 1143-1154出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr050455t
关键词
iTRAQ; quantitative proteomics; TGF-beta; lung cancer; epithelial-mesenchymal transitions; cell-migration; filamin; transglutaminase; HSPB1; beta 1-integrin
资金
- NCI NIH HHS [R01 CA094121, R01 CA094121-05, R01 CA094121-04] Funding Source: Medline
- NCRR NIH HHS [P41 RR18627-01] Funding Source: Medline
- NHLBI NIH HHS [HL57243, P050HL60289] Funding Source: Medline
Transforming growth factor-beta (TGF-beta) induces epithelial-mesenchymal transition (EMT) of epithelial cells in both normal embryonic development and certain pathological contexts. Here, we show that TGF-beta induced-EMT in human lung cancer cells (A549; adenocarcinoma cells) mediates tumor cell migration and invasion phenotypes. To gain insights into molecular events during EMT, we employed a global stable isotope labeled profiling strategy using iTRAQ reagents, followed by 2DLC-MS/MS, which identified a total of 51 differentially expressed proteins during EMT; 29 proteins were up-regulated and 22 proteins were down-regulated. Down-regulated proteins were predominantly enzymes involved in regulating nutrient or drug metabolism. The majority of the TGF-beta-induced proteins (such as tropornyosins, filamin A, B, & C, integrin-beta 1, heat shock protein27, transglutaminase2, cofilin, 14-3-3 zeta, ezrin-radixin-moesin) are involved in the regulation of cell migration, adhesion and invasion, suggesting the acquisition of a invasive phenotype.
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