期刊
JOURNAL OF MICROSCOPY
卷 222, 期 -, 页码 118-123出版社
WILEY
DOI: 10.1111/j.1365-2818.2006.01579.x
关键词
confocal microscopy; fluorescence microscopy; single molecule spectroscopy; TIR microscopy
类别
Epifluorescence, confocal and total internal reflection microscopy are the most widely used techniques for optical single-molecule experiments. Employing these methods, we recorded the emission intensity of the same single molecule as a function of the excitation rate under otherwise identical experimental conditions. Evaluation of these data provides a quantitative comparison of the signal-to-background ratios that can be achieved for the three microscopic techniques.
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