期刊
JOURNAL OF CLINICAL IMMUNOLOGY
卷 26, 期 3, 页码 204-212出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10875-006-9012-8
关键词
collagen-induced arthritis; T cell receptor V beta 3; type II collagen; CD4(+) T cell
类别
To investigate type II collagen (CII)-specific CD4(+) T cell receptors involving in Collagen-induced arthritis (CIA) in DBA/1J mice as a model of rheumatoid arthritis in humans, TCRV beta usage in draining lymph nodes (dLNs) was assessed by flow cytometric analysis at 3, 5, and 8 weeks after bovine CII immunizations. In the early stage of CIA, the draining lymph node CD4(+) T cells from CIA mice showed a higher proportion of CD4(+) V beta 3(+)subsets compared with those from control mice. The CD4(+) V beta 3(+) T cells were specifically and primarily expanded by antigen-specific stimulation in in vitro culture of dLNs lymphocytes and splenocytes from CIA mice. In addition, CII-reactive response was observed when CD4(+) V beta 3(+) T cells were added to a non-responding T cell population. The adoptive transfer of CD4(+) V beta 3(+) T cells produced exaggerated arthritis compared with that in the control group. Our results indicate that CD4(+)V beta 3(+) T cells, which were selectively expanded in dLN of CIA mice, play a pivotal role in CIA pathogenesis.
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