期刊
BIORESOURCE TECHNOLOGY
卷 97, 期 7, 页码 957-960出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2005.02.017
关键词
Burkholderia cepacia; phosphate solubilizing; bacteria; solubilization mechanisms; gluconic acid
Burkholderia cepacia CC-Al74 with a high ability for solubilizing tricalcium phosphate (TCP) was used to study the P-solubilization mechanism. We collected filtrates able to solubilize TCP from the cultures of strain CC-Al74 and demonstrated that the P-solubilization increased from 0 mu g ml(-1) to 200 mu g ml(-1) during exponential growth, when the pH decreased from 8 to 3. HPLC-analysis revealed that the solubilization of TCP was mainly caused by the release of 16.3 mM gluconic acid. At this concentration, gluconic acid was capable of solubilizing 376 mu g ml(-1) of TCP whereas water at pH 3 only solubilized 35 mu g ml(-1). The difference is due to the final H+ concentrations which were 13.5 mM and 1 mM in 16.3 mM gluconic acid and deionized water, respectively at pH 3. (c) 2005 Elsevier Ltd. All rights reserved.
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