期刊
ANALYTICAL CHEMISTRY
卷 78, 期 9, 页码 3084-3089出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0519460
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资金
- NHGRI NIH HHS [1R01 HG002644-01A1] Funding Source: Medline
- NIGMS NIH HHS [T32 GM07616] Funding Source: Medline
Single-cell gene expression analysis holds great promise for studying diverse biological systems, but methodology to process these precious samples in a reproducible, quantitative, and parallel fashion remains challenging. Here, we utilize microfluidics to isolate picogram and subpicogram mRNA templates, as well as to synthesize cDNA from these templates. We demonstrate single-cell mRNA isolation and cDNA synthesis, provide quantitative calibrations for each step in the process, and measure gene expression in individual cells. The techniques presented here form the foundation for highly parallel single-cell gene expression studies.
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