期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 168, 期 5, 页码 1452-1463出版社
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2006.050782
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资金
- NCI NIH HHS [CA-94595, R01 CA094595] Funding Source: Medline
- NHLBI NIH HHS [R01 HL062569, HL-38578, HL-62569, R01 HL038578, HL-38621, R37 HL062569, R01 HL064365, HL-64365, R01 HL038658, R01 HL038621, HL-38658] Funding Source: Medline
- NIDCR NIH HHS [R01 DE010742, R01 DE014183, R01-DE-10742, R01-DE-14183] Funding Source: Medline
Hypoxic stress activates various signal transduction pathways including posttranstational modification with the ubiquitin-like SUMO protein (SUMOylation). However, the molecular mechanisms by which SUMOylation regulates hypoxic responses remain unclear. Here, we investigated the ability of rat salivary Pa-4 epithelial cells to resist cell injury elicited by 1% O-2- or hypoxia-mimetic desferroxamine (DFO)-stimulated SUMOylation processes. By using Pa-4 cells stably transduced with lenti-SUMO-1 and a cell-permeant peptide harboring SUMO-binding motif to interfere with SUMO-dependent protein-protein interactions, we demonstrate that SUMOylation augments cell survival against DFO treatment. This appeared to be partly mediated through attenuation of Protein Kinase C (PKC)-delta activation and caspase-3 cleavage, hallmarks of pro-apoptotic signaling. Intriguingly, DFO-induced phosphorylation of DNA damage marker ataxia-telangiectasia-mutated protein S1981 preceded activation of PKC delta and caspase-3. Constitutive SUMOylation facilitated 1% O-2- or DFO-induced nuclear factor kappa B transactivation, possibly via activation of genotoxic signaling cascade. In addition, we observed transient preservation of transepithelial electrical resistance during the early stage of hypoxia (1% O-2) as well as enhanced transepithelial electrical resistance recovery after prolonged hypoxia in SUMO-1-expressing cell monolayers. In conclusion, our results unveil a previously unrecognized mechanism by which SUMOylation and activation of ataxia-telangiectasia-mutated protein, PKC delta, caspase-3, and nuclear factor kappa B signaling pathways modulate salivary adaptive responses to stress in cells exposed to either 1% O-2 or DFO.
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