期刊
NATURE IMMUNOLOGY
卷 7, 期 5, 页码 498-506出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ni1327
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资金
- NCI NIH HHS [T32 CA70083, CA48126, T32 CA070083] Funding Source: Medline
- NIAID NIH HHS [AI48125, AI12184, AI56296, P01 AI056296, R37 AI012184, R01 AI048126, R01 AI048125] Funding Source: Medline
The observation that the T-bet transcription factor allows tissue-specific upregulation of intracellular osteopontin (Opn-i) in plasmacytoid dendritic cells (pDCs) suggests that Opn might contribute to the expression of interferon-alpha (IFN-alpha) in those cells. Here we show that Opn deficiency substantially reduced Toll-like receptor 9 (TLR9)-dependent IFN-alpha responses but spared expression of transcription factor NF-kappa B-dependent proinflammatory cytokines. Shortly after TLR9 engagement, colocalization of Opn-i and the adaptor molecule MyD88 was associated with induction of transcription factor IRF7-dependent IFN-alpha gene expression, whereas deficient expression of Opn-i was associated with defective nuclear translocation of IRF7 in pDCs. The importance of the Opn-IFN-alpha pathway was emphasized by its essential involvement in cross-presentation in vitro and in anti herpes simplex virus 1 IFN-alpha response in vivo. The finding that Opn-i selectively coupled TLR9 signaling to expression of IFN-alpha but not to that of other proinflammatory cytokines provides new molecular insight into the biology of pDCs.
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