期刊
VACCINE
卷 24, 期 18, 页码 3793-3803出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2005.07.026
关键词
vaccines; Campylobacter; PEB1
We describe the evaluation of three live, attenuated Delta phoP/Q Salmonella enteric serovar Typhimurium strains expressing PEB1 minus its signal sequence (PEB1-ss) from three different plasmids: a pBR-based asdplasmid, an arabinose-based runaway plasmid, which each expressed PEB1-ss in the bacterial cytosol, and a PEB1::HlyA fusion plasmid that directs secretion of PEB1-ss into the extracellular milieu. Serum IgG responses specific for PEB1-ss were induced by pBR-derived and runaway plasmids, with 100 and 90% seroconversion, respectively, at a 1:500 dilution of anti-sera as measured by Western blot analysis, while the PEB1-ss::HlyA fusion plasmid induced serum IgG in only 20% of the mice. Although significant levels of anti-PEB serum IgG were induced, no protection against oral Campylobacter jejuni challenge was observed. (c) 2005 Elsevier Ltd. All rights reserved.
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