期刊
FERTILITY AND STERILITY
卷 85, 期 5, 页码 1391-1397出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2005.10.046
关键词
SR-BI; IVF; E-2; granulosa cells; HDL
资金
- NCRR NIH HHS [M01-RR-02179] Funding Source: Medline
- NHLBI NIH HHS [R01 HL075646] Funding Source: Medline
Objective: To determine the expression of the scavenger receptor, class B, type I (SR-BI) in human granulosa cells. Design: Prospective cohort analysis. Setting: Tertiary academic university hospital. Patient(s): Women undergoing IVF treatment. Intervention (s): Granulosa cells were isolated from oocyte retrievals. Main Outcome Measure(s): Total RNA and cell lysates were isolated from the granulosa cells. and SR-BI RNA and protein expression were quantified by real-time polymerase chain reaction and western blotting, respectively. Baseline and peak-E-2 levels were drawn before IVF treatment and before hCG Stimulation. Result(s): The expression of SR-BI RNA and protein were positively correlated. The prevalence of low SR-BI RNA expression (defined as below 25th percentile) was 26%, whereas the prevalence of high SR-BI expression (defined as above 75th percentile) was 24%. Expression of SR-BI RNA was not correlated with body mass index, age, race or ethnicity, primary diagnosis of infertility, or pregnancy outcomes. However, baseline and peak E-2 levels were significantly lower in subjects with low SR-BI RNA expression as compared with subjects with high SR-BI expression (P <.04). There was a positive correlation between the number of retrieved (P <.02) and fertilized oocytes (P <.002) and SR-BI RNA levels. Conclusion(s): Scavenger receptor-BI is expressed in human granulosa cells. Subjects with low SR-BI expression had lower baseline and peak E-2 levels and lower number of retrieved and fertilized oocytes.
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