Two novel species, Lysobacter daejeonensis sp nov and Lysobacter yangpyeongensis sp nov., isolated from Korean greenhouse soils

Two bacterial strains were isolated from greenhouse soils of Daejeon and Yangpyeong regions in Korea. The strains, designated GH1-9T and GH19-3(T), were Gram-negative and aerobic, with rod-shaped cells. Their DNA G + C contents were 61(.)7 and 67(.)3 mol%, respectively. The major fatty acids of strain GH1-9(T) were iso-C-16:0, iso-C-15:0, iSO-C-14:0, iSO-C-17:1 omega 9c and iso-C-11:0 3-OH and the major components of strain GH19-3(T) were iso-C-16:0, iso-C-15:0, C-16:1 omega 7c alcohol, iso-C-17:1 omega 9c and iso-C-11:0 3-OH. None of the species of the genus Lysobacter with validly published names showed 16S rRNA gene sequence similarity values of more than 97% with respect to the novel isolates. The closest sequence similarity of strain GH1-9T was with Lysobacter concretionis DSM 16239(T) (96-4%), whereas strain GH 19-3(T) showed the highest sequence similarity with Lysobacter enzymogenes DSM 2043(T) (96.6%). Polyphasic taxonomic studies indicated that the two strains should be classified as representing novel members of the genus Lysobacter. The names Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov. are proposed, with strains GH1-9(T) (= KACC 11406(T) = DSM 17634(T)) and GH 19-3(T) (=KACC 11407(T) =DSIM 17635(T)), respectively, as the type strains.