期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 39, 期 1-4, 页码 156-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2006.01.036
关键词
high-throughput screenings nitrilase; UV-vis spectroscopy; enantioselectivity
In this paper we describe a colorimetric method for the screening of nitrilases. When a buffered solution of CoCl2 is added to a nitrilase-catalyzed hydrolysis reaction, the ammonia product forms a complex with the cobalt ion resulting in a color change from light pink to yellow, which can readily be quantified using a spectrophotometer at 375 nm. This method has been demonstrated for both wild-type and evolved nitrilases. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据