期刊
ANALYTICA CHIMICA ACTA
卷 566, 期 2, 页码 167-171出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2006.03.018
关键词
hippuric acid; methylhippuric acid; mandelic acid; phenylglyoxylic acid; gas chromatography-mass spectrometry; urine samples
A sensitive and precise method for the simultaneous determination of hippuric acid, o-, in- and p-methylhippuric acids, mandelic acid and phenylglyoxylic acid, which are major urinary metabolites of toluene, o-, in- and p-xylenes, styrene and ethylbenzene, respectively, was developed. These metabolites were converted into their methyl ester derivatives with methanol in hydrochloric acid, and then quantitated by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring using a DB-1 capillary column. The injected compounds were quantitatively and reproducibly resolved within 19 min with a detection limit of 8-27 pg. The calibration curves were linear in the range of 0.05-25 mu g for each compound, with correlation coefficients above 0.9999. This rnethod was successfully used to analyze small amounts of both rat and human urine samples without any interference frorn coexisting substances. Overall recoveries of these compounds spiked in urine samples were 92-104%. The analytical results of the contents of these metabolites in the rat and human urine samples are presented. (c) 2006 Elsevier B.V. All rights reserved.
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