期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 94, 期 1, 页码 118-127出版社
WILEY
DOI: 10.1002/bit.20835
关键词
shear rate; apoptosis-like cell death; (CLP)-L-3; DNA fragmentation; siROS; NADH oxidase
Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of a caspase-3-like protease ((CLP)-L-3) followed by DNA fragmentation. Shear stress leads to an increase in specific intracellular reactive oxygen species (siROS), possibly through activation of NADH oxidase (NOX). The formation of siROS precedes the activation of (CLP)-L-3 and DNA fragmentation, thus establishing siROS as the molecular link between shear stress and apoptosis-like cell death. A model is proposed in which NOX is viewed as being strategically placed on the plasma membrane of B. subtilis that senses and converts a mechanical force arising from shear stress into a chemical signal leading to activation of (CLP)-L-3, DNA fragmentation, and thus, apoptosis-like cell death. (c) 2006 Wiley Periodicals, Inc.
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