期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 94, 期 1, 页码 151-158出版社
WILEY
DOI: 10.1002/bit.20836
关键词
plasmids; vectors; libraries; bacteria
资金
- NIAID NIH HHS [R21 AI055773-01] Funding Source: Medline
- PHS HHS [A1056687] Funding Source: Medline
We describe the construction of 36 stable vectors for genomic library construction in gram-negative species. These vectors contain the pBBR1 replicon that has been shown to stably replicate in every gram.-negative species tested. The plasmids also contain bidirectional, rho-independent transcriptional terminators flanking the multiple cloning site, which allows for greater insert stability, and thus, greater genomic representation. Each vector varies in its antibiotic resistance cassette, mobilization function, and promoter used to express insert sequences. These vectors should prove useful in the screening of highly representational genomic libraries in a broad variety of gram-negative species. (c) 2006 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据