IR, Raman and surface-enhanced Raman study of desferrioxamine B and its Fe(III) complex, ferrioxamine B

IR, Raman and surface-enhanced Raman scattering (SERS) spectroscopies were employed for the structural investigation of the chelator desferrioxamine B and its Fe(HI) complex, ferrioxamine B. Comparing the FT-IR absorptions of desferrioxamine B and ferrioxamine B, the structural changes due to the Fe(HI) chelation were clearly evidenced by spectral analysis. Despite the intense C=O stretching vibration in the FT-Raman spectrum of desferrioxamine B, the FT-SERS spectrum is dominated by a strong C=N stretching vibration, indicating a delocalization of the N lone pair of electrons and the formation of the C=N bond in the hydroxamate groups. The desferrioxamine B and ferrioxamine B molecules are adsorbed to the silver colloidal particles through the 0 atoms of the hydroxamate functional groups, with similar orientation with respect to silver surface. (c) 2005 Elsevier B.V. All rights reserved.