期刊
CHEMPHYSCHEM
卷 7, 期 5, 页码 1112-1118出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200500660
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Currently, there is great interest in the development of methods suitable for determining the stoichiometry of biomolecules attached to nanoparticles. We describe the use of the dynamic light-scattering technique (DLS) to determine the stoichiometry of the protein cytochrome P450(BS beta) attached to CdS and CdSe quantum dots (QDs). The enzyme-conjugated (DLS) have different diffusion kcharacteristics compared to the QD and enzyme precursors, expressed in their size, scattering intensity as well as zeto-potential values. The significant enhancement of the scattering intensity of QDs observed upon conjugation with the P450(BS beta) due to the refractive-index increment and the systematic variation in zeta potential resulting from charge neutralization of the anionic QDs by the cationic histidine-tagged P450(BS beta) have been used for stoichiometry determination.
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