期刊
GENES & DEVELOPMENT
卷 20, 期 10, 页码 1294-1307出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1422006
关键词
mass spectrometry; proteomics; ribosome; Saccharomyces cerevisiae; translation
资金
- NCI NIH HHS [CA098131, P50 CA098131] Funding Source: Medline
- NHLBI NIH HHS [P01 HL068744, HL68744] Funding Source: Medline
- NIAID NIH HHS [N01AI40079, T32 AI049824, T32 AI49824] Funding Source: Medline
- NIEHS NIH HHS [R01 ES011993, ES11993] Funding Source: Medline
- NIGMS NIH HHS [GM68900, R01 GM064779, R01 GM068900, GM64779] Funding Source: Medline
Translation regulation is a critical means by which cells control growth, division, and apoptosis. To gain further insight into translation and related processes, we performed multifaceted mass spectrometry-based proteomic screens of yeast ribosomal complexes and discovered an association of 77 uncharacterized yeast proteins with ribosomes. Immunoblotting revealed an EDTA-dependent cosedimentation with ribosomes in sucrose gradients for 11 candidate translation-machinery-associated (TMA) proteins. Tandem affinity purification linked one candidate, LSM12, to the RNA processing proteins PBP1 and PBP4. A second candidate, TMA46, interacted with RBG1, a GTPase that interacts with ribosomes. By adapting translation assays to high-throughput screening methods, we showed that null yeast strains harboring deletions for several of the TMA genes had alterations in protein synthesis rates (TAM7 and TAM19), susceptibility to drugs that inhibit translation (TAM7), translation fidelity (TMA20), and polyribosome profiles (TAM7, TAM19, and TMA20). TMA20 has significant sequence homology with the oncogene MCT-1. Expression of human MCT-1 in the Delta tma20 yeast mutant complemented translation-related defects, strongly implying that MCT-1 functions in translation-related processes. Together these findings implicate the TMA proteins and, potentially, their human homologs, in translation related processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据